Preliminary list of host genes significant in large fusiform rust galls vs. small fusiform rust galls

 

Arrays of 323 genes (redundant #) were probed with RNA from xylem or phloem isolated from either small or large galls

No multiple testing corrections performed – therefore, at a=0.1 and 323 genes, we expect 32 genes to be significant by chance.

Although 11 of the putatively significant host genes were also significant in the Fusarium experiment, 9 of them were regulated in opposite directions

 

significance test:

Type 2 = large (Lg) vs small (Sm) galls

Type 3 = large phloem (LG P) vs small phloem (Sm P)

Type 4 = large xylem (Lg X) vs small xylem (Sm X)

 

Significance test
clone treatment Assigned ‘functions’
(E value <10-5)		 Type 2
Lg vs Sm		 Type 3
Lg P vs Sm P		 Type 4
Lg X vs Sm X		 Direction of Regulation l significantly up-regulated by Fusarium
pi129 salicylate CCoAOMT 1 1 down-reg in Lg P
pi231 F.R. suscept. p3H6 1 1 down-reg Lg X
pi213 P.C. resist. PAL 1 1 down-reg in Lg X
pi240 P.C. resist. phenylcoumaran benzylic ether reductase 1 1 1 down-reg in Lg P and Lg X
catechol-OMT 1 1 down-reg in Lg X
pi287 P.C. suscept. ABC transporter-like protein 1 1 up-reg Lg P l
pi140 salicylate putative ARF6 1 down-reg in Lg
H12 healthy putative MADS box protein 1 1 down-reg in Lg P
MAPK2 regulatory 1 down-reg in Lg
Myb 126 regulatory 1 up-reg in Lg P
pi318 F.R. suscept. RNA binding domain protein 1 1 down-reg lg P
pi314 F.R. suscept. RNA binding protein 1 down-reg in Lg
H37 healthy callose synthase 1 1 down-reg in Lg P
pi305 P.C. suscept. Catalase isozyme 2 1 down-reg in Lg
pi203 P.C. resist. putative gamma-thionin precursor 1 1 down-reg Lg X
pi185 P.C. resist. putative LRR protein 1 down-reg in Lg
pidd1 wound class I chitinase 1 1 1 down-reg Lg P and Lg X l
pi279, pi303, pi304 P.C. suscept. class III peroxidase 1 down-reg Lg X l
pi73 salicylate class IV chitinase 1 down-reg in lg
pi326 F.R. suscept. DNA-damage-repair/toleration protein 1 1 1 up-reg Lg P and Lg X
H7 healthy heat shock protein 1 1 down-reg in Lg X
pi253 P.C. suscept. embryo abundance protein EMB20 1 1 down-reg Lg P
pi164 P.C. resist. embryonic abundant protein EMB32 1 1 1 down-reg in Lg P and Lg X
H25 healthy Metallothionein-like protein EMB30 1 1 1 down-reg in Lg P and Lg X
H13 healthy endoxyloglucan transferase 1 1 down-reg in Lg P
H17 healthy glyceraldehde 3 -P dehydrogenase 1 down-reg in Lg
G40 galled glyceraldehde 3 -P dehydrogenase 1 1 down-reg in Lg P
H23 healthy MtN3-like 1 1 1 down-reg in Lg P and Lg X
pi193 P.C. resist. NADH dehydrogenase 1 1 down-reg in Lg P
pic54 chitosan NADH dehydrogenase 1 1 down-reg Lg P
H24 healthy NADH dehydrogenase 1 1 down-reg in Lg P
H19 healthy NAM 1 1 down-reg in Lg P
pi317 F.R. suscept. Ribose 5-P isomerase 1 1 down-reg Lg X
pi284 P.C. suscept. ribosomal protein L1 1 1 up-reg Lg P l
G37 galled ribosomal protein L17 1 1 up-reg in Lg P
J6 F.R. suscept. tubulin alpha 1 chain 1 up-reg in lg P
G12 healthy No similaritity 1 up-reg in Lg
G36 healthy No similaritity 1 up-reg in Lg P
G39 healthy No similaritity 1 down-reg in Lg
pi204 PC R No similaritity 1 down-reg in Lg
pi228a F.R. suscept. No similaritity 1 down-reg in Lg l
pi311a F.R. suscept. No similaritity 1 up-reg in Lg
pi97 salicylate No similaritity 1 down-reg Lg
H1 healthy No similaritity 1 down-reg in Lg P
H41 healthy No similaritity 1 up-reg in L P
pi233 F.R. suscept. No similaritity 1 down-reg Lg X
G50 galled No similaritity 1 1 up-reg in Lg P
G73 galled No similaritity 1 1 down-reg in Lg P
G75 galled No similaritity 1 1 down-reg in Lg P
pi205 P.C. resist. No similaritity 1 1 down-reg in Lg P
pi226 F.R. suscept. No similaritity 1 1 down-reg Lg P
pi252 P.C. suscept. No similaritity 1 1 down-reg Lg P
pi260 P.C. suscept. No similaritity 1 1 up-reg Lg P
pidd13 wound No similaritity 1 1 down-reg Lg P
H35 healthy No similaritity 1 1 down-reg in Lg P
G41 galled No similaritity 1 1 1 up in Lg P and Lg X
G45 galled No similaritity 1 1 1 up in Lg P and Lg X
G56 galled No similaritity 1 1 1 up in Lg P and Lg X
G65 galled No similaritity 1 1 1 up in Lg P and Lg X
G72 galled No similaritity 1 1 1 down-reg in Lg P and Lg X
H34 healthy No similaritity 1 1 1 down-reg in Lg P and Lg X
pi235 F.R. suscept. No similaritity 1 1 1 up in Lg P and Lg X
pi245 F.R. suscept. No similaritity 1 1 1 no signal in large galls….
pi261 P.C. suscept. No similaritity 1 1 1 up in Lg P and Lg X
pi266 P.C. suscept. No similaritity 1 1 1 down-reg Lg P and Lg X
pi323 F.R. suscept. No similaritity 1 1 1 up Lg P and Lg X
H6 healthy No similaritity 1 1 down-reg in Lg X
pi173 P.C. resist. No similaritity 1 1 down-reg in Lg X
pi306 P.C. suscept. No similaritity 1 1 down-reg Lg X
pi280 P.C. suscept. No similaritity 1 1 down-reg Lg X l
G51 galled pine EST 1 1 up-reg in Lg P
H10 healthy pine EST 1 down-reg in Lg
H14 healthy pine EST 1 1 down-reg in Lg P
H21 healthy pine EST 1 1 down-reg in Lg P
H32 healthy pine EST 1 down-reg in Lg X
H38 healthy pine EST 1 1 1 down-reg in Lg P and Lg X
J4 F.R. suscept. pine EST 1 1 up-reg in Lg P
J7 F.R. suscept. pine EST 1 1 up-reg in Lg P
pi121 salicylate pine EST 1 1 down-reg in Lg P
pi150, pi125 salicylate pine EST 1 1 down-reg in Lg P
pi139 salicylate pine EST 1 1 down-reg in Lg P l
pi166, pi167 P.C. resist. pine EST 1 1 down-reg in Lg P
pi174 P.C. resist. pine EST 1 1 down-reg in Lg X
pi188 P.C. resist. pine EST 1 down-reg in Lg P
pi192 P.C. resist. pine EST 1 1 down-reg in Lg P
pi194, pi196 P.C. resist. pine EST 1 1 down-reg in Lg X
pi197 P.C. resist. pine EST 1 1 down-reg in Lg X
pi210, pi211, pi212 P.C. resist. pine EST 1 1 down-reg Lg P
pi237 F.R. suscept. pine EST 1 1 down-reg Lg X
pi264 P.C. suscept. pine EST 1 1 down-reg Lg X
pi277 P.C. suscept. pine EST 1 down-reg in Lg l
pi310 SA pine EST 1 1 1 down-reg in Lg P and Lg X
pi76 salicylate pine EST 1 down-reg in Lg l
pi90 salicylate pine EST 1 1 down-reg Lg P
pic56 chitosan pine EST 1 down-reg Lg
pidd6 wound pine EST 1 down-reg Lg P l
pi130 salicylate plant EST 1 down-reg in Lg l
G11 galled plant EST 1 up-reg in Lg P
G54 galled plant EST 1 1 down-reg in Lg P
G55 galled plant EST 1 1 up in Lg P
H11 healthy plant EST 1 1 1 down-reg in Lg P and Lg X
H39 healthy plant EST 1 1 1 down-reg in Lg P and Lg X
H5 healthy plant EST 1 1 down-reg in Lg X
pidd18 wound unclassified 1 down-reg Lg
G53 galled unclassified 1 1 up-reg in Lg P
H33 healthy unclassified 1 1 down-reg in Lg P
H22 healthy unclassified 1 1 down-reg in Lg X
pi291 P.C. suscept. unclassified 1 1 down-reg Lg X
pi319 F.R. suscept. unclassified 1 1 down-reg Lg X
pi77 salicylate unclassified 1 1 down-reg Lg X
J5 F.R. suscept. unclasssified 1 1 down-reg in Lg X
pic23 chitosan unclasssified 1 1 down-reg Lg P